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KMID : 0984920170190020043
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Journal of Skin Barrier Research
2017 Volume.19 No. 2 p.43 ~ p.44
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Holotomography (HT) Techniques for Non-Invasive Label-Free 3D Imaging of Live Cells And Tissues
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Park Yong-Keun
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Abstract
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Holotomography (HT) uses a laser interferometry to measure 3-D refractive index (RI) distribution. HT servses as a powerful tool for imaging small transparent objects, such as biological cells and tissues. HT is an optical analogous to X-ray computed tomography (CT); HT measured multiple 2-D holograms of a sample with various illumination angles, from which a 3-D RI distribution of the sample is reconstructed by inversely solving the wave equation.
Unlike conventional fluorescence-based imaging techniques, HT provides label-free 3-D imaging capability. Without any fixation or labeling, 3-D images of live cells can be obtained with high spatial resolution (down to 110 nm). Furthermore, HT provide quantitative imaging capability : RI maps of a cell are precisely and quantitative measured, from which various cellular analysis can be followed. In this talk, we will present the recently developed 3-D holotomography setup using a dynamic mirror device. In particular, we will discuss the principle of HT techniques and the previous application in the field of hematology, cell biology, neuroscience, and infectious diseases1-4. The outcome demonstrates outstanding visualization of 3D refractive index maps of live cells, which will be potentially used in various applications in biology and medicine. we will also discuss about the commercialzation of the technique.
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KEYWORD
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Quantitative phase imaging, Optical diffraction tomography, Holotomography, Label-free imaging, 3D microsc
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